Pollen morphological study in subfamily Papilionoideae using Confocal Laser Scanning Microscopy

Anjali Trivedi; Alka Srivastava; Anjum Farooqui; Salman Khan; Anil K. Pokharia; David K. Ferguson; Veeru Kant Singh

doi:10.54991/jop.2022.538

Authors

Anjali Trivedi Birbal Sahni Institute of Palaeosciences, 53, University Road, Lucknow 226007, India
Alka Srivastava 2Dayanand Girls Post–Graduate College, 13/394, Parwati Bagla Road, Kanpur 208001, India
Anjum Farooqui Birbal Sahni Institute of Palaeosciences, 53, University Road, Lucknow 226007, India
Salman Khan Birbal Sahni Institute of Palaeosciences, 53, University Road, Lucknow 226007, India
Anil K. Pokharia Birbal Sahni Institute of Palaeosciences, 53, University Road, Lucknow 226007, India
David K. Ferguson Department of Palaeontology, University of Vienna, Althanstrasse 14, Vienna A–1090, Austria
Veeru Kant Singh Birbal Sahni Institute of Palaeosciences, 53, University Road, Lucknow 226007, India

DOI:

https://doi.org/10.54991/jop.2022.538

Keywords:

CLSM, Pollen morphology, Papilionoideae, Kanpur, India, Cluster Analysis

Abstract

The pollen morphological study was carried out in the subfamily Papilionoideae using Confocal Laser Scanning Microscopy (CLSM) to facilitate the identification of pollen in sedimentary archives. Pollen has long been used as an excellent proxy for understanding past vegetation, ecology, climate and agricultural strategies of ancient settlements and therefore, its identification at a specific level is of utmost importance. The modern pollen samples were retrieved from plants growing in urban and rural areas of Kanpur city, Uttar Pradesh, India. The cluster analysis and PCA of pollen morphological characters in the subfamily determine the generic and species relationships outlining the affinity of taxa in the subfamily Papilionoideae.

सारांश

तलछटी अभिलेखागार में पराग की पहचान के लिए कन्फोकल लेजर स्कैनिंग माइक्रोस्कोपी (सीएलएसएम) का उपयोग करते हुए उपपरिवार पैपिलिओनोइडी में पराग रूपात्मक अध्ययन किया गया। प्राचीन बस्तियों की पुरावनस्पति, पारिस्थितिकी, जलवायु और कृषि रणनीतियों को समझने के लिए पराग का लंबे समय से एक उत्कृष्ट प्रतिनिधि के रूप में उपयोग किया जा रहा है और इसलिए, एक विशिष्ट स्तर पर इसकी पहचान अत्यंत महत्वपूर्ण है। आधुनिक पराग के नमूने कानपुर, उत्तर प्रदेश के शहरी और ग्रामीण क्षेत्रों में उगने वाले पौधों से प्राप्त किए गए थे। क्लस्टर विश्लेषण और पराग रूपात्मक वर्णों का पीसीए उपपरिवार पैपिलिओनोइडी के टैक्सा की आत्मीयता को रेखांकित करते हुए जेनेरिक और प्रजाति संबंधों को निर्धारित करता है।

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